Kaffir Lime (Citrus hystrix DC.) Juice as an Alternative to Glacial Acetic Acid in Turk’s Solution for Leukocyte Counting
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Abstract
Manual leukocyte counts are still often used in remote areas, due to limited tools or reagents. Turk solution is a diluting solution used in leukocyte examination, composed of glacial acetic acid with a level of 22,176 g/L which lyses erythrocytes and gentian violet which colors leukocytes. Kaffir lime (Citrus hystrix DC) is one of the natural ingredients that contains citric acid and is able to lyse erythrocytes. The purpose of this study was to determine whether the variation of kaffir lime juice (Citrus hytsrix DC) is effective as a substitute for glacial acetic acid in turk solution. This research is experimental with total sampling technique. The samples used were venous blood of male students of the Nasional College of Health Sciences' Bachelor of Applied Physiotherapy Study Program batch 2021-2023. The data were analyzed with the One Way ANOVA test, the sig results were obtained 0,001 (p < 0.05) in all groups, which means that there is a significant difference between the number of leukocytes counted using turk solution, kaffir lime juice concentration of 1% and 2%. So that kaffir lime juice solution (Citrus hystrix DC) is not effective as a substitute for glacial acetic acid in turk solution.
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