Determination of Total Flavonoid Content of A Peel Ethyl Acetate Extract of Carica papaya L

Abstract

Flavonoids are secondary metabolites of polyphenols, found widely in plants and food and have various bioactive effects. one of which is found in the skin of papaya (Carica papaya L.). Flavonoids are semi-polar compounds. Semi-polar solvents such as ethyl acetate. Therefore, the purpose of this study was to use ethyl acetate as a solvent to determine the levels of flavonoids in the skin of papaya (Carica Papaya L.).Papaya (Carica papaya L.) peel extract was obtained by maceration method with ethyl acetate as solvent. Qualitative analysis using Wilstater test, Bate-smith test, and NaOH test. Quantitative test using UV-Vis spectrophotometer.Secondary metabolites in papaya (Carica Papaya L.) rind showed positive compounds in the flavonoid test. While the test for alkaloids and saponins showed negative results and did not contain compounds. From the calculation results, the average level of quercetin equivalence was 27.0242 ppm ±0.2168 while the total flavonoid content of the ethyl extract of papaya fruit peel (Carica papaya L.) was 2.7023% ± 0.021.The results of phytochemical screening of ethyl acetate extract of papaya (Carica papaya L.) peel contained flavonoid compounds. And the total flavonoid content of papaya peel acetate extract was 2.7023%